Figure 4

Treatment of KCI-18 kidney tumors with genistein and radiation. Panel A: Design of X-ray irradiation of tumor-bearing kidney. Mice in 3 jigs were positioned on an aluminum frame mounted on the X-ray machine. Exposure of the right kidney is confirmed by metallic clips surgically placed in the kidney and visualized in double exposure X ray radiograph while rest of their body was shielded with lead shield positioned above the mice. Panel B: Treatment schedule diagram. On day 12–14 after KCI-18 cell injection in kidney, mice were treated daily with oral genistein at 5 mg/day/mouse. On day 15, established renal tumors were irradiated with 8 Gy photons. One day later, genistein treatment was resumed and given every other day. On day 28, mice were killed and tumors and metastatic tissues were resected. Panel C: Response of primary kidney tumors to radiation and genistein. Weights of the tumor-bearing kidneys and their median weight, from 7–9 mice per treatment group, are reported in boxplot showing the range of data. Panel D: Response of mesentery metastases. Mesentery nodules were prominent along the intestines and enumerated. Each dot represents the number of metastatic nodules per mouse. Panel E: Response of lung metastases. White metastatic nodules were enumerated on lungs insufflated with India ink and bleached as described in Materials and Methods. Each dot represents the number of nodules per mouse. Data from four experimental treatment groups are shown: mice treated with vehicle (Control), genistein only (Gen), radiation only (Rad), genistein combined with radiation (Gen+Rad).