14-3-3σ protein expression in hematological malignancies. Western blot analysis of whole lysates (20 μg/lane) of clinical specimens of hematological malignancies was done with anti-14-3-3σ monoclonal antibody. A representative result is shown with HeLa cells (2 μg/lane) as a control. Anti-α-tubulin antibody was used to show the protein loading. 14-3-3σ mRNA expression levels determined by real-time RT-PCR and standardized to that of Jurkat cells are shown below.