Figure 1

Expression of 14-3-3σ mRNA in hematological malignancies and cell lines. Expression levels of 14-3-3σ mRNA were determined by real-time RT-PCR and standardized by that of Jurkat cells (an open square). Dotted line denotes mean plus 2 SDs (2.17) of controls and a mean for each disease or cell line is shown as a horizontal bar. The vertical scales are different between the lower and higher levels. Open circles denote patients with p53 mutations identified by RT-PCR-SSCP analysis. Controls include 5 PBMNC, 3 BMMNC, 2 reactive lymph nodes; CML, 7 patients with blast crisis (BC) and 1 patient with chronic phase (CP) of chronic myeloid leukemia; AML, 12 patients with acute myeloid leukemia; ALL, 16 patients with acute lymphoid leukemia including 3 patients with Burkitt leukemia/lymphoma; ATL, 8 patients with adult T-cell leukemia/lymphoma (1 chronic, 1 acute, and 6 lymphoma variants); CLL, 5 patients with chronic lymphocytic leukemia/small lymphocytic lymphoma and 3 patients with prolymphocytic leukemia; DLBCL, 27 patients with diffuse large B-cell lymphoma; FL, 29 patients with follicular lymphoma; other NHL, 4 patients with T-cell lymphoma, 2 patients with mantle cell lymphoma and 3 patients with unclassified lymphoma; PCD, 12 patients with plasma cell dyscrasias including 1 patient with plasma cell leukemia, 4 patients with Waldenström's macroglobulinemia and 7 patients with multiple myeloma; cell lines include 14 myeloid, 15 T-cell, and 10 B-cell lines (all listed in reference [23]) and an epithelial cell line, HeLa (an open triangle). Asterisks denote statistical significance by Scheffe test after combining CML BC, AML and ALL as a group of acute leukemia and DLBCL, FL and other NHL as a group of NHL: * P < 0.05, ** P < 0.01.