Figure 3

TGZ inhibition of cell spreading. ES-2 cells treated with DMSO or 20 μM TGZ were plated on coverslips which were coated with FN (10 μg/ml) or poly-L-lysine (25 μg/ml, PLL). The cell surface area after 1 h was determined using MetaMorph software on at least 100 cells from five different fields (100 × magnification); n = 3 experiments. *P < 0.005 versus control. Right panels represent phase-contrast photographs of ES-2 cells treated with DMSO or TGZ for 1 h.