Figure 2

Chetomin (CHT) inhibits the hypoxic activation of hypoxia-responsive-element-(HRE-) mediated EGFP fluorescence in stably transfected HT 1080 human fibrosarcoma cells in a dose and incubation-time dependent manner. After chetomin pre-treatment for two or four hours, HT 1080 cells were cultured for 12 hours under hypoxia resulting in total chetomin treatment times of 14 or 16 h as indicated. A maximum suppression of EGFP fluorescence was achieved when cells were treated with 150 nM chetomin for 16 hours (representative FACS experiment). Higher doses had no additional effect.