Polymorphic microsatellite marker analysis of Hone1, #3/Hone1 and 5 derived tumors. PCR products of genomic DNA amplification using 2 polymorphic microsatellite markers D3S1588 and D3S1289, located within 3p21.1 (54.1 Mb) and 3p14.3 (54.5 Mb), respectively, were analyzed using semi-automated ABI Prism™ 3100 Genetic Analyzer for capillary electrophoresis. The results are presented in graphical view according to GeneScan and GenoTyper 2.1 software. Recipient Hone1, donor MCH903.1, hybrid #3/Hone1 and 5 tumors derived from the #3/Hone1 were genotyped. Horizontal lines above the peaks show the original ratio between peak heights (characteristic for the #3/Hone1). Arrows indicate relative reduction of endogenous (derived from Hone1) peaks in the tumor compared to the #3/Hone1 used for SCID mice inoculations. This reduction was detected for both markers in two independent experiments.