Alpha smooth muscle actin (SMA) and cytokeratin expression. SMA (green staining) was detected in LM-234mf (A) but not in LM-234ep (C) cells, using a FITC-conjugated primary antibody. Nuclei (blue staining) were identified using DAPI. Cytokeratin was undetectable in LM-234mf (B), whereas strongly expressed in LM-234ep (D), as revealed by immunohistochemistry using an anti-Pan cytokeratin antibody. Magnification bar, 100 μm. Cell lysates (25 μg/lane) were analyzed using Western blot for cytokeratins (E) and αSMA (F). The cytokeratins detected with the anti-pan antibody used range between 40 and 68 kDa. The band immunodetected for αSMA has an approximate molecular weight of 42 kDa. Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) was used as a loading control.