Figure 4From: The isothiocyanate class of bioactive nutrients covalently inhibit the MEKK1 protein kinase Synthesis of Bio-ITC. Norbiotinamine hydrochloride was converted to the isothiocyanate, Bio-ITC, by mild conditions with 1,1'-thiocarbonyldi-2,2'-pyridone (TCDP) in the presence of an amine base (Et3N). Synthesis and characterization are described in detail in the materials and methods.Back to article page