Immunofluorescence of 14-3-3ζ protein in oral cancer cells, HSC2. Cells were fixed and immunolabeled with anti-14-3-3ζ antibody followed by streptavidin-conjugated-FITC labeled secondary antibody (Green fluorescence) and nuclei were counterstained with propidium iodide (red fluorescence). (a) Nuclei showing red fluorescence of Propidium Iodide. (b) Green fluorescence in cytoplasm and nucleus of HSC2 cells. (c) Merged figure showing localization of 14-3-3ζ protein in oral cancer cells (a-c, original magnification × 200).