TP53 exon 5 amplicon redesign allowed detection of the BT-20 645A>C mutation. Cell line mutant samples are in red and wild-type samples are in blue. All plots shown in this figure are difference plots. Panel A; BT-20 394A>C and SKBR3 524G>A mutations with the original amplicon designed for exon 5. Panel B; BT-20 394A>C mutation is now detectable with the 5a amplicon. Panel C; SKBR3 524G>A mutation with the 5b amplicon.