Figure 1

HIF-1α expression in BHY and FaDu cell lysates. Cells were maintained under normoxia for 24 h (24hN) or hypoxia for 2 (2hH), 8 (8hH), 16 (16hH) and 24 h (24hH). Cells treated with 100 μM desferroxamine (DFO) for 24 h under normoxic conditions served as positive controls. 5 μg of each cell lysate was loaded per well and visualized on western blots using HIF-1α or β-actin antibodies as indicated.