Real-time PCR for MIF mRNA expression in bladders from WT mice. Mice were euthanized after treatment with or without BBN for the weeks indicated. The treated to control ratio was calculated by dividing each RQ (relative quantification) value in the BBN group by the mean of the corresponding control group. Each symbol is the mean ± SD for 2–3 mice per group. All RQ values were calculated after normalization to GAPDH.