Correlations of EGFR mutations and increases in EGFR and HER2 copy number to gefitinib response in a retrospective analysis of lung cancer patients

Table 4 EGFR and HER2 copy number alterations

#	Sex	Ethnicity	Smoking Status	Histology	Source Tissue	Block Type¹	Response²	EGFR Mutation	EGFR/CEP7	HER2/CEP17	EGFR Stratification³	HER2 Stratification³
9	F	Asian	N	adeno.	Cerebellum	Tissue Block	SD	Not Sequenced	2.1	1.9	High Poly.	Low Poly.
11	F	Asian	Unk.	adeno.	Lung	Tissue Block	PR	Exon 19 Del*, Exon 20 V774L	2.7	1.5	High Poly.	High Trisomy
27	F	Caucasian	Y	SCC	Lung	Tissue Block	PD	None	2.1	1.5	High Poly.	Low Poly.
34	M	Caucasian	Y	SCC	Lung	Tissue Block	SD	None	17.3	2.6	Gene Amp.	High Poly.
36	M	Asian	Y	adeno.	Pleura	Tissue Block	SD	None	2.0	2.0	Low Poly.	Low Poly.
40	M	Caucasian	Y	adeno.	Pleura	Cell Block	SD	None	3.1	1.4	High Poly.	Low Poly.
42	M	Caucasian	Y	adeno.	Lymph Node	Tissue Block	Unk.	None	2.1	2.3	Low Poly.	High Poly.
43	F	Caucasian	Y	adeno.	Lymph Node	Tissue Block	PD	None	2.7	0.9	High Poly.	Low Trisomy
44	F	Asian	N	adeno.	Pleura	Tissue Block	PR	Exon 20 S768I, Exon 20 L815L	1.9	2.9	Low Poly.	High Poly.
56	M	Caucasian	Y	adeno.	Lung	Tissue Block	Unk.	None	1.5	2.0	High Trisomy	Low Poly.
57	M	Caucasian	Y	adeno.	Lymph Node	Tissue Block	SD	None	1.6	2.2	Low Poly.	High Poly.
64	F	Caucasian	Y
			Pre Rx:	adeno.	Lymph Node	Tissue Block	-	None	1.0	1.2	Low Trisomy	High Trisomy
			Post Rx:	adeno.	Pericaridium	Tissue Block	SD	None	2.2	1.3	Low Poly.	Low Trisomy
66	F	Asian	N	adeno.	Lung	Tissue Block	PR	Exon 19 Del*	2.9	1.2	High Poly.	Low Trisomy

Patient data provided for samples displaying increased EGFR or HER2 copy number (Probe/CEP ratio > 2.0) or identified as FISH+ (High Polysomy or Gene Amplification)
* = no mutations detected in normal tissue remaining after microdissection
1 = source of patient material (Tissue Block = microdissected formalin-fixed paraffin-embedded tissue block; Cell Block = whole section or microdissected formalin-fixed paraffin-embedded cell block; Cytology = scraped cytology slide)
2 = response as measured radiographically and defined by SWOG modification of the WHO criteria [20].
PD = progressive disease, SD = stable disease, PR = partial response, Unk. = Unknown
3 = Copy number stratification as proposed by Cappuzzo et al [13]. (Disomy = < 2 copies in > 90% of cells, Low Trisomy = ≤2 copies in ≥40% of cells, 3 copies in 10–40% of cells, ≥4 copies in < 10% of cells, High Trisomy = ≤2 copies in ≥40% of cells, 3 copes in ≥40% of cells, ≥4 copes in < 10% of cells, Low Polysomy: ≥4 copies in 10–40% of cells, High Polysomy = ≥4 copies in 40% of cells, Gene Amplification = ≥15 copies in ≥10% of cells)

ISSN: 1471-2407