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Table 1 Growth inhibition evaluation of troxacitabine and gemcitabine in four pancreatic cell lines.

From: Synergistic activity of troxacitabine (Troxatyl™) and gemcitabine in pancreatic cancer

Cell Line GI50 (nM) ± SEM CIa
  Gemcitabine Troxacitabine  
Panc-1 4 ± 0.7 40 ± 10 0.41
MIA PaCa-2 13 ± 3 330 ± 20 0.71
AsPC-1 10 ± 2 4 ± 1 0.37
Capan-2 70 ± 20 150 ± 30 0.52
  1. a Fa denotes fraction affected (e.g., Fa of 0.5 is equivalent to a 50% reduction in cell growth). CI >1 denotes antagonism, CI = 1 denotes additivity, and CI < 1 denotes synergism.
  2. Cells were exposed to graded concentrations of troxacitabine or gemcitabine either alone or in combination at a ratio of 1:100 gemcitabine vs. troxacitabine for 72 h as shown in Fig. 1. Values for GI50 (± SEM) (the drug concentration that inhibited cell growth by 50% as determined from concentration-effect relationships using GraphPad Prism version 3.0 software) from three experiments, each conducted with three replicates, were calculated from non-linear regression analysis of data plotted as percentages of control values against the logarithm of drug concentrations. CI (combination index) values at a Fa value of 0.5 were generated from the data of Fig. 2 with the CalcuSyn, version 2.0 software.