Cell Line | GI50 (nM) ± SEM | CIa
|
|---|
| | Gemcitabine | Troxacitabine | |
|---|
Panc-1 | 4 ± 0.7 | 40 ± 10 | 0.41 |
MIA PaCa-2 | 13 ± 3 | 330 ± 20 | 0.71 |
AsPC-1 | 10 ± 2 | 4 ± 1 | 0.37 |
Capan-2 | 70 ± 20 | 150 ± 30 | 0.52 |
-
a Fa denotes fraction affected (e.g., Fa of 0.5 is equivalent to a 50% reduction in cell growth). CI >1 denotes antagonism, CI = 1 denotes additivity, and CI < 1 denotes synergism.
- Cells were exposed to graded concentrations of troxacitabine or gemcitabine either alone or in combination at a ratio of 1:100 gemcitabine vs. troxacitabine for 72 h as shown in Fig. 1. Values for GI50 (± SEM) (the drug concentration that inhibited cell growth by 50% as determined from concentration-effect relationships using GraphPad Prism version 3.0 software) from three experiments, each conducted with three replicates, were calculated from non-linear regression analysis of data plotted as percentages of control values against the logarithm of drug concentrations. CI (combination index) values at a Fa value of 0.5 were generated from the data of Fig. 2 with the CalcuSyn, version 2.0 software.
