Table 1 Primers and probes used in real-time RT-PCR
Gene | Primer sequence | Product size |
|---|---|---|
SCGB2A2 | 5'-GAACACCGACAGCAGCA-3' 5'-TCTCCAATAAGGGGCAGCC-3' | 104 bp |
SCGB1D2 | 5'-CTGAGCTCACAGCAAAAC -3' 5'-GAGCTGGGCAGAAC-3' | 105 bp |
GAPDH | 5'-GAAGGTGAAGGTCGGAGTC-3' 5'-GAAGATGGTGATGGGATTTC-3' | 226 bp |
Gene | TaqMan probe | |
SCGB2A2 | 5'-TGGTCCTCATGCTGGCGGCC-3' | |
SCGB1D2 | 5'-CCATGAAGCTGTCGGTGTGTCTCCTG-3' | |
GAPDH | 5'-CAAGCTTCCCGTTCTCAGCC-3' |