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Table 2 Design of primers and TaqMan probes for allelic discrimination assay.

From: Polymorphisms of the XRCC1, XRCC3 and XPDgenes and risk of colorectal adenoma and carcinoma, in a Norwegian cohort: a case control study

Primer /Probe Sequence
XRCC1 codon 194
   Forward primer: 5'-GAG GAT GAG AGC GCC AAC TC-3'
   Reverse primer: 5'-TCA CTC AGG ACC CAC GTT GTC-3'
   Probe C allele: 5'-VIC-TCT TCA GCC GGA TCA-MGB/NFQ-3'
   Probe T allele: 5'-FAM-TCT TCA GCTGGA TCA A-MGB/NFQ-3'
XRCC1 codon 280
   Forward primer: 5'-GGT GCT AAC CTA ATC TAC TCT TTG TCT TC-3'
   Reverse primer: 5'-TGG GCT CGG TGC CTT CT-3'
   Probe G allele: 5'-VIC-AGC TCC AAC TCG TAC C-MGB/NFQ-3'
   Probe A allele: 5'-FAM-AGC TCC AAC TCA TAC C-MGB/NFQ-3'
XRCC1 codon 399
   Forward primer: 5'-GAG TGG GTG CTG GAC TGT CA-3'
   Reverse primer: 5'-CAT TGC CCA GCA CAG GAT AA-3'
   Probe G allele: 5'-VIC-CCT CCC GGA GGT AA-MGB/NFQ-3'
   Probe A allele: 5'-FAM-CCT CCC AGA GGT AAG-MGB/NFQ-3'
  1. The bold underlined letters represent the polymorphic positions.