Figure 4
Effect of zoledronate on GGPP/GGOH incorporation into proteins in vitro (A) and in vivo (B). A: Geranyl-geranyl transferase I (GGTase I) was quantified using an in vitro test based on [3H]-prenyl pyrophosphate (0.5 μM, 8–10 Ci/mmol) incorporation into a mutant form of H-ras with a geranyl-geranylation CAXX box. The level of prenylation is expressed as a percentage of maximum incorporation of [3H]-prenyl, as determined by allowing the uninhibited reaction to go to completion. B: Western-blot analysis; PC-3 cells are treated by vehicle, ZOL 20 μM; ZOL 20 μM + FOH 10 μM; ZOL 20 μM + GGOH 10 μM.