Figure 1

Schematic representation of inverse correlation between promoter methylation and expression of CCNA1 in CC cell lines. (A) Diagram of methylated and non-methylated sequences after bisulfite modification covering the area of both primers (underlined) in the promoter region of CCNA1. M, DNA size marker. Top panel, 10-bp ladder; bottom four panels, 100-bp ladder. Neg, negative. (B) Duplex MSP analysis of cell lines. Upper and lower arrows indicate non-methylated and methylated amplicons, respectively. MSP, methylation-specific PCR. (C-E) RT-PCR of the CCNA1 gene after 28, 30 and 35 cycles, respectively. (F) RT-PCR of the GAPDH gene as an internal control.