Figure 6

Effect of PPARγ ligands on apoptosis. The effect of PPARγ ligands on apoptosis was measured using caspase-3/7 activation (A) and DNA fragmentation (B). A, HL60 and TSU-Pr1 cells were treated with either control (0.1% DMSO), Doxorubicin (0.5 or 1 μM; Dox). TSU-Pr1 cells were also treated with troglitazone (50 and 100 μM; TGZ), 15-deoxy-prostaglandin J₂ (5.6 and 10 μM; 15dPGJ2) or rosiglitazone (10 μM; RGZ) for 48 hrs. Levels of activated caspase-3/7 were determined by fluorescence measured at 535 nm. Values are mean ± SEM of 2 experiments performed in triplicate. B, HL60 and TSU-Pr1 cells were treated with Doxorubicin (1 μM; Dox), troglitazone (100 μM; TGZ), 15-deoxy-prostaglandin J₂ (10 μM; 15dPGJ2) or rosiglitazone (10 μM; RGZ) for 72 hrs. DNA was extracted and run on a 1 % agarose gel. Statistical significance * P < 0.01, different to TSU-Pr1 control (ANOVA followed by Dunnett post test).