Software cross-section through a cell with nestin-positive nucleus. Nestin occurrence in cell nuclei was confirmed by confocal microscopy using software cross-section (B–D) through the selected cell (A, the plane of software cross-section is highlighted by the red line). The signal for nestin was detected also in the cell nucleus (C). The position of nucleoli is indicated by arrowheads. Nestin (A, C–D, green) stained by indirect immunofluorescence using FITC-labeled secondary antibody; nuclei labeled by DAPI (A–B, D, blue) are shown; bars, 20 μm (A), 10 μm (B–D).