High resolution melting analysis for the rapid and sensitive detection of mutations in clinical samples: KRAS codon 12 and 13 mutations in non-small cell lung cancer

Table 2 Summary of results for 30 lung cancers screened for mutations in KRAS

Patient	Histology	HRM	Sequencing	AA change	EGFR sequencing
1	Adenocarcinoma	wt	wt	-	wt
2	Adenocarcinoma	wt	wt	-	wt
3	Adenocarcinoma	mut	35G > T	G12V	wt
4	Adenocarcinoma	mut	34G > T	G12C	wt
5	Adenocarcinoma	wt	wt	-	wt
6	Adenocarcinoma	mut	35G > A	G12D	wt
7	Adenocarcinoma	wt	wt	-	wt
8	Adenocarcinoma	wt	wt	-	wt
9	Adenocarcinoma	wt	wt	-	exon 19 del
10	Adenocarcinoma	wt	wt	-	exon 19 del
11	Adenocarcinoma	wt	wt	-	wt
12	Adenocarcinoma	wt	wt	-	wt
13	Adenocarcinoma	mut	35G > T	G12V	wt
14	Adenocarcinoma	mut	34G > T	G12C	wt
15	Adenocarcinoma	wt	wt	-	wt
16	Adenocarcinoma	wt	wt	-	wt
17	Adenocarcinoma	wt	wt	-	exon 19 del
18	Adenocarcinoma	mut	38G > A	G13D	wt
19	Adenocarcinoma	wt	wt	-	wt
20	Adenocarcinoma	wt	wt	-	wt
21	Adenocarcinoma	wt	wt	-	wt
22	Adenocarcinoma	mut	35G > T	G12V	wt
23	Large cell	wt	wt	-	Not done
24	Large cell	wt	wt	-	Not done
25	Large cell	mut	35G > C	G12A	Not done
26	Large cell	wt	wt	-	Not done
27	Large cell	wt	wt	-	Not done
28	Large cell	wt	wt	-	Not done
29	Large cell	mut	35G > T	G12V	Not done
30	Large cell	wt	wt	-	Not done

HRM – high resolution melting, wt – wild-type, mut – mutant, AA change – amino acid change, exon 19 del – EGFR Exon 19 deletion