Effects of PD0166285 on cyclin D transcription. A: RT-PCR analysis of cyclin D mRNA in 0.5 μM PD0166285 treated cells. RT yielded a cDNA template for PCR amplification with specific-specific primers. GAPDH expression was analyzed as a control. B: Quantitative real-time PCR analysis of specific mRNA after treatment with 0.5 μM PD0166285. Three independent experiments were carried out.