Effects of PD0166285 on the cellular location of Wee1 and adhesion. A: After 4 h of PD0166285 treatment, cells were fixed and doubly stained for immunofluorescence with PI (red) and anti-Wee1 (green). B: After 4 h of 0.5 μM PD0166285 treatment, morphologic changes and double stained with anti-α-tubulin (green) and anti-Wee1 (red) were observed. Shown are typical images obtained by laser confocal microscopy. Bar: 10 μm.