Intracellular localization of TLR4 in NB-1 cells. A, NB-1 cells were stained with no antibody, PE-conjugated isotype match IgG or PE-conjugated anti-TLR4 antibody. B, Permeabilized NB-1 cells were stained with the same protocol. The fluorescent histogram was analyzed by a laser flow cytometer. Data is the representative of at least three independent experiments.