Table 4 PCR conditions for RT-PCR amplification
From: FGFR1 and WT1 are markers of human prostate cancer progression
GENE | N° of cycles | Denaturation | Annealing | Extension |
|---|---|---|---|---|
WT1 | 35 | 94°C/30 sec | 64°C/30 sec | 72°C/30 sec |
MART1 | 30 | 94°C/1 min | 60°C/1 min | 72°C/2 min |
TACC1 | 30 | 94°C/1 min | 55°C/1 min | 72°C/2 min |
ACTIN | 25 | 94°C/30 sec | 55°C/30 sec | 72°C/30 sec |