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Table 4 PCR conditions for RT-PCR amplification

From: FGFR1 and WT1 are markers of human prostate cancer progression

GENE N° of cycles Denaturation Annealing Extension
WT1 35 94°C/30 sec 64°C/30 sec 72°C/30 sec
MART1 30 94°C/1 min 60°C/1 min 72°C/2 min
TACC1 30 94°C/1 min 55°C/1 min 72°C/2 min
ACTIN 25 94°C/30 sec 55°C/30 sec 72°C/30 sec