Table 3 Primers and conditions for RT-PCRs.
From: Frequent promoter hypermethylation of RASSF1A and CASP8 in neuroblastoma
Gene | Primer forward (5'–3') | Primer reverse (5'–3') | Size (bp) | T (°C) | Cycles |
|---|---|---|---|---|---|
RASSF1A | TCTGTGGCGACTTCATCTGG | TTGGGCAGGTAAAAGGAAGT | 424 | 60 | 35 |
NORE1A | ATCCAGTTGGACTGCAGTCA | GGGATTGTCCACAACCATG | 450 | 60 | 35 |
BLU | GCTTAGCACACACAACCTGC | CCTTGGCAAAACTTGTGAGG | 233 | 67 | 30 |
CASP8 | AGAGAAGCAGCAGCCTTGAAGG | AGACAGTATCCCCGAGGTTTGC | 160 | 60 | 35 |
TFR | GTCAATGTCCCAAACGTCACCAGA | ATTTCGGGAATGCTGAGAAAACAGACAGA | 298 | 60 | 30 |