Table 2 Primers and conditions for MSPs.
From: Frequent promoter hypermethylation of RASSF1A and CASP8 in neuroblastoma
Gene | Primer forward (5'–3') | Primer reverse (5'–3') | Size (bp) | T (°C) | Cycles |
|---|---|---|---|---|---|
RASSF1A | |||||
- U | GAGAGTGTGTTTAGTTTTGTTTTTG | CCCATACTTCACTAACTTTAAACAC | 183 | 62 | 38 |
- M | GAGAGCGCGTTTAGTTTCGTTTTC | ACCCGTACTTCGCTAACTTTAAACG | 184 | 58 | 35 |
NORE1A | |||||
- U | ATTTATATTTGTGTAGATGTTGTTTGGTAT | ACTTTAACAACAACAACTTTAACAACTACA | 215 | 54 | 40 |
- M | CGTCGTTTGGTACGGATTTTATTTTTTTCGGTTC | GACAACTTTAACAACGACGACTTTAACGACTACG | 202 | 62 | 40 |
BLU | |||||
- U | TTTGTGGGTTATAGTTTGAGAAAGTG | AACAAATTAACCACACCTACAC | 156 | 60 | 30 |
- M | TTCGTGGGTTATAGTTCGAGAAAGCG | AACGAATTAACCGCGCCTACGC | 156 | 66 | 30 |
CASP8 | |||||
- U | TAGGGGATTTGGAGATTGTGA | CCATATATCTACATTCAAAACAA | 320 | 53 | 40 |
- M | TAGGGGATTCGGAGATTGCGA | CGTATATCTACATTCGAAACGA | 321 | 50 | 40 |