Microarray-based identification and RT-PCR test screening for epithelial-specific mRNAs in peripheral blood of patients with colon cancer

Table 2 Primers used for RT-PCR

Primer	Sequence (5' → 3')	Direction	Nt 5'^a	Expected size (bp)
PRSS22	ctatcatcgactcggaagtc	f	668	676
	gatatgtgggcagggttaca	r	1343
RANBP3	gctcgtacgagtgtagacactgggc	f	2195	191
	agggacctcgacagagctgtgcct	r	2385
TMEM69	gatgtctctccagcaaaacttttc	f	281	299
	ctggctccataagccatctgag	r	579
B2M	gcgggcattcctgaagctgac	f	25	586
	tacatcaaacatggagacagcac	r	610

^a Nt 5' indicates the position of the nucleotide (Nt) at the 5' end of the primer. [GenBank:AB021288 (B2M), AB010779 (PRSS22), NM_003624 (RANBP3), NM_016486 (TMEM69)]. B2M, β-2 microglobulin; f, forward; r, reverse; bp, base pair.

ISSN: 1471-2407