Figure 2From: Relevance of cyclin D1b expression and CCND1polymorphism in the pathogenesis of multiple myeloma and mantle cell lymphoma Cyclin D1a and cyclin D1b protein expression. A. Whole cell extracts were obtained from CD19+ cells of MCL patients, separated on SDS-PAGE and sequentially immunoblotted with anti-cyclin D1a (sc-718) or anti-cyclin D1b (R3) Abs. For loading controls, blots were also revealed with anti-β-tubulin Ab (sc-9104, not shown). B. Proteins were obtained from MCL cell lines and analyzed as described in A except that we used DCS-6 anti-cyclin D1 Ab which recognizes both isoforms.Back to article page