Figure 5
Validation of oligonucleotide microarray results of 8 selected genes by semi-quantitative RT-PCR. HeLaT-β-catenin-RNAi cells were treated with or without DOX for 3 days. RT-PCR was performed as described in Methods. Amplified fragments of CTNNB1, MYBL2, BAG3, PTEN, PDCD6IP, HIF1A, BAG2 and DAP3 genes are indicated. β-actin gene was used as an internal control and was amplified from the same samples.