Figure 1
From: Heparin (GAG-hed) inhibits LCR activity of Human Papillomavirus type 18 by decreasing AP1 binding
GAG-hed affects growth rates in a HeLa tumoral model induced in athymic female mice. Nude female mice were subcutaneously inoculated on the dorsal part with HeLa cells (12 mice total, three mice per group); each animal received only one cell injection (1 × 106 cells in suspension, in a total volume of 20 μl). On established tumours, GAG-hed doses applied were: 0.08 mg/dose, group A; 0.8 mg/dose, group B; and 8 mg/dose, group C. Control animals were injected with saline solution and are denoted as group D. Panel A; Data showing the average tumour volume determined from day 16 after cell inoculation (day taken as starting point for treatment) to day 30th. Tumour volume was determined by two dimensions measurement: width and length. Z axis: average of total tumour volume (expressed in mm3) of all animals (n = 3); Y axis: days after HeLa cells inoculation; X axis: applied GAG-hed treatment. (B) Comparison and statistic analysis values at day 30th under differential dose application. *p < 0.05, **p < 0.005, Student's t-test in comparison to saline treated control.