Figure 4

Expression of met in prostate cancer cell lines. (A) Met RT-PCR detection of six transcripts in prostate cell lines. Amplified fragments of control μ-globulin and the cytoplasmic domain of human met are shown on an ethidium-bromide stained agarose gel. The length of the h-met PCR product was 262 bp. Product levels were close to undetectable in LNCaP and C4-2 samples. (B) Western blot analysis of total cell lysates from same prostate cell lines and HeLa cells. Equal amounts of cell lysate were separated and immunoblotted with antibodies against either the extracellular (B) or cytoplasmic (C) domains of Met. Met was detected by characteristic double bands in all cell lines except LNCaP and C4-2.