Table 3 Secondary data analysis and DNA micro arrays. Secondary data analysis was performed on data published by Dhanasekaran et al. [19] to identify adhesion molecules the expression that are altered during prostate cancer progression. Comparisons were made between normal (norm) tissue/benign prostate hyperplasia (BPH) and cancer (CaP) and metastasis (Met). The normalized mean and standard deviation values were evaluated for significance with Instat 4.0 (GraphPAD software) using one-way analysis of variance (ANOVA) are presented, with the level of significance set at p < 0.05 (*). To determine which of the genes are responsive to CXCL12, RNA was analyzed from CXCL12 treated LNCaP and LNCaP C4-2B cells (200 ng/ml, 2 hours) were compared against non-treated cells by DNA micro array using the Human genome U133 gene chips (Affymetrix Corp. Santa Clara, CA). Later, fold change values were determined using DNA-Chip (dChip version 1.1) [26].

2.34

0.86

1.19

0.76

1.97

0.84

1.04

0.68

1.19

1.47

2.76

0.20*

1.01

0.27*

2.80

0.27*

-1.09

1.17

-1.38

0.65

3.07

0.27

1.11

0.35

2.87

0.38

-1.19

0.57

-1.06

0.76

1.00

0.37

3.50

1.01

1.00

0.82

-1.03

0.95

-1.12

1.23

2.11

0.22

1.12

0.21

1.90

0.27

-1.07

0.68

1.01

0.91

3.24

0.24

1.05

0.24*

3.15

0.25

-1.38

0.60

1.26

0.93

3.56

0.15

1.20

0.16

2.94

0.16

-1.65

0.85

1.21

0.62

1.96

0.21

1.32

0.33*

1.47

0.37*

1.19

1.07

1.02

0.47

2.17

0.43*

0.56

0.45

3.84

0.24

-1.05

0.85

-1.02

1.06

3.31

0.28

1.04

0.25

3.14

0.23

-1.43

0.55

1.02

0.31

3.83

0.25

1.27

0.26*

2.98

0.23*

1.19

0.54

-1.25

0.64

2.10

0.18

0.88

0.16

2.36

0.17*

1.10

0.92

-1.14

0.81

2.42

0.50

0.68

0.52*

3.58

0.28*

1.32

0.65*

1.95

0.49*

3.01

0.24

1.18

0.30

2.61

0.34*

-3.61

2.14

-2.23

0.36

1.72

0.60*

0.43

0.70*

4.09

0.34*

-1.05

1.20

-1.20

0.62

2.06

0.52*

0.53

0.56*

3.93

0.26

1.10

1.84

-1.11

0.97

2.46

0.15

1.08

0.15

2.37

0.12

-1.07

1.00

1.06

0.37*

3.22

0.24*

0.62

0.27

4.53

0.21

-1.04

0.73

-1.34

0.59

2.60

0.20

1.18

0.22

2.19

0.19

1.09

1.11

1.01

1.11

2.09

0.41

0.48

0.32

4.12

0.23

1.14

0.51

1.01

0.87

2.10

1.53

0.35

1.40

5.45

0.16*

1.19

0.44*

1.37

0.29

3.48

0.15

0.90

0.16

3.82

0.91

-1.17

0.68

1.24

0.53

4.36

0.24

0.94

0.28

4.32

0.28

-1.48

0.37

1.17

0.46

3.15

0.25

0.96

0.26

3.26

0.25

1.08

0.83

-1.02

0.67

1.60

1.22

0.33

1.27

4.86

0.30

1.03

0.83

1.10

0.67

2.16

0.34*

0.59

0.37*

3.66

0.17*

-1.12

1.40

1.01

0.76

3.34

0.31*

1.16

0.30

2.95

0.33

1.01

1.21

1.13

0.84

2.34

0.20*

0.91

0.24

2.62

0.23

1.24

1.43

-1.02

0.83