Figure 7

Human prostate tissue samples stained with CD164. High-density tissue micro arrays of human CaP tumors were immunostained for CD164, AR and PSA as described in the methods. Immunostaining intensity was scored by a genito-urinary pathologist: as absent (1), weak (2), moderate (3) or strong (4). Scoring was performed blindly using a telepathology system without knowledge of overall the tumor grade, tumor size or clinical outcome [30]. Representative staining of CD164 staining for (A) benign prostate hyperplasia (B) localized prostate cancer (C) metastatic prostate cancer to bone marrow. Correlation of CD164 staining with prostate specific antigen (PSA) staining intensity (D), androgen receptor (E) and tissue type (F). The data demonstrate that CD164 staining intensity increases with increasing tumor grade.