Table 1 Primers and PCR conditions used in BACH1 mutation analysis.
Exon | Strand | Primer name | Nucleotide sequence (5'→3') | Amplicon lenght (bp) | Annealing temperature (°C) |
|---|---|---|---|---|---|
2 | Forward | Ex2F | CTGTTTCCAGATTTCTCCC | Â | Â |
| Â | Reverse | Ex2R | GTGAACCCAGAAAATATTCTCC | 331 | 58 |
3 | Forward | Ex3F | CCCTGGAGTGCAATCTCACT | Â | Â |
| Â | Reverse | Ex3RN | TAGCGACAGCATGGCTGAA | 319 | 48 |
4 | Forward | Ex4F | CCTGGGTGAACTGGGCTGTAG | Â | Â |
| Â | Reverse | Ex4RN | TAACAGTAATAATTAAGACTC | 316 | 48 |
5 | Forward | Ex5FN | TTGCCTACCTGTAAGTTATTTATG | Â | Â |
| Â | Reverse | Ex5RN | ACCATGTTCAGCTGTAACTAACTG | 233 | 60 |
6 | Forward | Ex6F | GAGCTGTTTTGGCCTTTGAGA | Â | Â |
| Â | Reverse | Ex6RN | CTGAGTGGGTTGCTACTGTCCT | 317 | 55 |
7 | Forward | Ex7F | GTTCTGATTCCATGTGAGGTT | Â | Â |
| Â | Reverse | Ex7R | GTACATATAAAACACATACTGAGT | 448 | 55 |
8 | Forward | Ex8F | GATGTTCCTCAAATTCTGAGATAA | Â | Â |
| Â | Reverse | Ex8R | CATCTAAAAGCTTTTACATTCAAC | 386 | 55 |
9 | Forward | Ex9F | GCCTATAGTGTGAATTTTAAAATG | Â | Â |
| Â | Reverse | Ex9R | CCTAGTTAACCAAAGTTTACTAAC | 395 | 55 |
10 | Forward | Ex10F | GATCAACGCATGACAATAATGATG | Â | Â |
| Â | Reverse | Ex10R | GGGTTACTCACTAGATTTAATCTG | 320 | 55 |
11 | Forward | Ex11F | GCATGTTTTGTTGGGTTTCATTGT | Â | Â |
| Â | Reverse | Ex11R | GGTATGTATTAAACACATGCTAGC | 326 | 55 |
12 | Forward | Ex12F | GTACCAGCTCTTTCAAATGAG | Â | Â |
| Â | Reverse | Ex12R | CTATCTTTAAAAGAGTCAACCAC | 360 | 55 |
13 | Forward | Ex13FN | GTGCTGGGATTACAGGTGTGAGCCA | Â | Â |
| Â | Reverse | Ex13RN | ACTTGCTGGCACTTCAGGTATCTTC | 317 | 60 |
14 | Forward | Ex14F | CTTGTTGCTTGATCTTTTATGTAC | Â | Â |
| Â | Reverse | Ex14R | CTAGGAAGCTTACTGTGGTAA | 356 | 55 |
15 | Forward | Ex15FN | ACAGCTCTATGAGATATATTG | Â | Â |
| Â | Reverse | Ex15RN | TCATAGGAGAACAAGTACAAT | 504 | 55 |
16 | Forward | Ex16FN | TTACTTAAAGACATTGAACTT | Â | Â |
| Â | Reverse | Ex16RNN | CACTATAAAAGCAAAGCGC | 392 | 54 |
17 | Forward | Ex17FN | CTGTTAGAAGTTAATATGATG | Â | Â |
| Â | Reverse | Ex17RN | GAATACATACCAGTTCCTATG | 325 | 55 |
18 | Forward | Ex18F | CCAATTTTCTGTCTGTCCCAC | Â | Â |
| Â | Reverse | Ex18R | GATAGTAGAGCTCATGTTATGTG | 254 | 55 |
19 | Forward | Ex19F | CTTCACTAGAAAAAGCAAGTG | Â | Â |
| Â | Reverse | Ex19R | CCACCATATTTAAGGAATTAATC | 523 | 48 |
20A | Forward | Ex20AFN | ACCTAGCAATTATGTTAGCT | Â | Â |
| Â | Reverse | Ex20ARN | TCTGTATCTTCAGGATCGTA | 584 | 48 |
20B | Forward | Ex20BFN | ATTGATGCCACCCTTACTA | Â | Â |
| Â | Reverse | Ex20BRNN | TAACATAAGCATGATGACATA | 565 | 54 |