Table 1 Candidate single nucleotide polymorphisms (SNPs) and their respective probes and primers
Gene | Location | SNP ID | Forward primer | Reverse primer | FAM probe | TET probe |
|---|---|---|---|---|---|---|
IL-1A | +4845 G>T | rs17561 | TGCACTTGTGATCATGGTTTTAGA | TCCTCATAAAGTTGTATTTCACATTGC | CAAGCCTAGGTCATCACCTTTTAGCTTCC | AAGCCTAGGTCAGCACCTTTTAGCTTCC |
IL-1B | -511 C>T | rs16944 | TTGAGGGTGTGGGTCTCTACCT | AGGAGCCTGAACCCTGCATAC | TTCTCTGCCTCGGGAGCTCTCTGT | TTCTCTGCCTCAGGAGCTCTCTGTCA |
IL-1B | +3954 C>T | rs1143634 | GCCTGCCCTTCTGATTTTATACC | CATCGTGCACATAAGCCTCGTTA | TTCAGAACCTATCTTCTTTGACACATGGGA | CAGAACCTATCTTCTTCGACACATGGGA |
IL-1RN | +2018 T>C | rs419598 | GGGATGTTAACCAGAAGACCTTCTATCT | CAACCACTCACCTTCTAAATTGACATT | AACAACCAACTAGTTGCTGGATACTTGCAA | ACAACCAACTAGTTGCCGGATACTTGC |
IL-4R | +1902 A>G | rs1801275 | AGGCTTGAGAAGGCCTTGTAA | CCGAAATGTCCTCCAGCAT | CATGTACAAACTCCTGATAGCCACTGGTG | CATGTACAAACTCCCGATAGCCACTGG |
IL-6 | -174 G>C | rs1800795 | GCTGATTGGAAACCTTATTAAGATTGT | AATGACGACCTAAGCTGCACTTT | ACGTCCTTTAGCATCGCAAGACACAAC | ACGTCCTTTAGCATGGCAAGACACAAC |
IL-10 | -1082 G>A | rs1800896 | GATAGGAGGTCCCTTACTTTCCTCTTA | CACACACAAATCCAAGACAACACTAC | CTACTTCCCCCTCCCAAAGAAGCCT | CCTACTTCCCCTTCCCAAAGAAGCC |