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Table 1 Placental immunohistochemical analysis of cleaved PARP, caspase-3 and cytosolic cytochrome-c (number of positive cells counted) and the ratios of cleaved PARP, caspase-3 and cytosolic cytochrome-c positive cells in the spongy trophoblast layer of tumour-bearing rats or rats injected with ascitic fluid compared to control rats at different stages of pregnancy (16th, 19th and 21st days). Polyclonal antibodies against PARP, caspase-3 and cytochrome-c (all from Santa Cruz Biotechnology; anti PARP and anti-cytochrome-c diluted 1:100; anti-caspase diluted 1:250) were detected with a secondary biotinylated anti-goat antibody (diluted 1:500) and reacted with DAB in the presence of H2O2. The cells were counted using Image ProPlus software.

From: Cancer during pregnancy alters the activity of rat placenta and enhances the expression of cleaved PARP, cytochrome-c and caspase 3

Number of positive cells counted Ratio between treated and control groups
  Cleaved PARP Caspase-3 Cytosolic cytochrome-c   Cleaved PARP Caspase-3 Cytosolic cytochrome-c
C16 43.1 ± 7.1a 3.7 ± 0.4a 3.4 ± 0.3a     
W16 136.7 ± 12.2b 16.0 ± 1.4c 4.7 ± 0.3a W16/C16 2.89 ± 0.30a 3.02 ± 0.37a 1.52 ± 0.11a
A16 71.7 ± 9.2c 7.9 ± 0.4c 6.7 ± 0.4c A16/C16 1.41 ± 0.13b 2.90 ± 0.35a 2.54 ± 0.25b
C19 48.0 ± 5.9a 1.3 ± 0.2b 4.9 ± 0.3a     
W19 149.2 ± 20.9b 9.3 ± 0.7c 13.3 ± 0.7b W19/C19 3.40 ± 0.23a 7.56 ± 0.74b 3.03 ± 0.20b
A19 115.9 ± 9.5b 4.6 ± 0.2a 15.4 ± 0.4d A19/C19 3.00 ± 0.30a 3.84 ± 0.34a 3.35 ± 0.21b
C21 54.8 ± 7.6a 2.5 ± 0.3a 4.8 ± 0.3a     
W21 148.0 ± 16.9b 7.3 ± 0.7c 12.7 ± 0.6b W21/C21 2.99 ± 0.54a 2.50 ± 0.70a 3.00 ± 0.22b
A21 73.4 ± 11.7c 7.2 ± 0.4c 10.2 ± 0.6b A21/C21 1.67 ± 0.36b 2.43 ± 0.50a 2.78 ± 0.29b
  1. Groups: control (C), tumour-bearing (W) and ascitic fluid-injected (A) rats. Twenty areas (300 μm2 each) were examined in one tissue section each from at least six rats per group. The cells were counted using Image ProPlus software. The results are expressed asthe mean ± SEM. Different superscript letters indicate significant differences within each column (P < 0.05).