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Table 2 List of genes identified in the microarray analysis between HeLa Tet-off cells harboring pTIG-TSPY and those harboring the vector pTIG alone

From: TSPY potentiates cell proliferation and tumorigenesis by promoting cell cycle progression in HeLa and NIH3T3 cells

Gene GenBank ID Expression Remarks
TSPY1 NM_003308 26.5 Testis specific protein, Y-linked, stably transfected gene in the cells
MAP2 NM_002374 3.41 Microtubule-associated protein 2, neurogenesis and tumorigenesis
RGC32 NM_014059 2.56 Response gene to complement 32, deregulation of cell cycle required for tumor cell growth
PDGFC NM_016205 2.32 Platelet derived growth factor C, a mitogenic factor
MAP1B NM_005909 1.93 Microtubule-associated protein 1B, structural protein, neural development, predictor for breast cancer
EFEMP1 NM_004105 1.79 EGF-containing fibulin-like extracellular matrix protein 1, growth factor
G1P2 NM_005101 1.72 α-interferon inducible protein, IFI-15K, facilitates viral growth
LUM NM_002345 1.62 Lumican, epithelial cell migration and tissue repair
NANOS1 NM_199461 1.57 Human nanos homolog (of Drosophila), germ stem cell development expressed in spermatogonia and spermatocytes in human testis
WNT5A NM_003392 1.55 Wingless-type MMTV integration site family member 5A, Wnt gene family, implicated in oncogenesis and embryogenesis
CD24 NM_013230 1.55 Small cell lung carcinoma cluster 4 antigen, high expression in SCLC
TMTC1 NM_175861 1.53 Transmembrane and tetratricopeptide repeats containing 1, multi-functional, possibly involved in regulation of cell cycle and/or mitosis.
CCND2 NM_001759 1.46 Cyclin D2 complexes with CDK4 or CDK6, G1/S transition, amplified and over-expresses in numerous types of tumors
RAP1A NM_002884 1.44 RAS oncogene family member
EGFR NM_005228 1.41 Epidermal growth factor receptor (ERBB oncogene), cell proliferation, amplified and expresses at high levels in many tumors
ANKRD15 NM_015158 1.38 Ankyrin repeat domain 15, tumor cell growth in renal cell carcinoma
GSPT1 NM_002094 1.32 G1 to S phase transition 1, an inhibitor of apoptosis
EP300 NM_001429 1.26 E1A binding protein p300, a co-factor for hypoxia inducible factor 1A
CDKN2B NM_004936 0.71 Cyclin-dependent kinase inhibitor 2B (p15), binds to and prevents CDK4/CDK6 activation and G1/S progression, tumor suppressor
CUL1 NM_003592 0.68 Cullin 1, involves in deneddylation and modulates G1/S transition
TGFB3 NM_003239 0.67 Transforming growth factor, b3, suppresses tumor formation and blocks cell cycle progression
PTN NM_002825 0.66 Pleiotrophin, neurite growth promoting factor 1, down-regulated in breast cancer
TIMP1 NM_003254 0.64 Tissue inhibitor of metalloproteinase 1, suppresses tumor growth and metastasis
DUSP5 NM_004419 0.58 Dual specificity phosphatase 5, inactivates kinases by dephosphorylation, and negatively regulates MAP kinases
SPARC NM_003118 0.56 Secreted protein, acidc cysteine-rich (osteonectin), loss of expression in lung cancers, regulates cell proliferation
CLU NM_001831 0.56 Clusterin, proapoptotic in colon cancer, down-regulated in CaP
IGFBP3 NM_000598 0.49 Insulin-like growth factor binding protein 3, pro-apoptotic, its inactivation or repression is essential for growth of various tumors
  1. Genes listed in boldface were analyzed with semi-quantitative for RT-PCR (Figure 6).