Table 2 Antibodies and immunohistological staining procedures
Antigen | Antibody | Source | Dilution | Detection | Pretreatment |
|---|---|---|---|---|---|
Vimentin | mAb V9 | DAKO, Glostrup, Denmark | 1:1000 | LSAB/ HRP | Steamer |
Ki-67 nuclear antigen | mAb MIB-1 | DAKO, Glostrup, Denmark | 1:100 | LSAB/AP | Steamer |
broad spectrum Cks | mAb KL-1 | Immunotech, Marseille, France | 1:50 | LSAB/ HRP | Steamer |
Ck5/6 | mAb D5/16B4 | Zymed, San Francisco, USA | 1:50 | LSAB/ AP | Steamer |
Ck14 | mAb Ll002 | dianova GmbH, Hamburg, Germany | 1:50 | LSAB/ AP | Steamer |
Ck8/18 | mAb 5D3 | dianova GmbH, Hamburg, Germany | 1:40 | LSAB/ AP | Steamer |
Ck19 | mAb RCK108 | DAKO, Glostrup, Denmark | 1:80 | LSAB/ AP | Steamer |
P63 | mAb 4A4 | DAKO, Glostrup, Denmark | 1:100 | LSAB/ AP | Steamer |
Sm-α-actin (SMA) | mAb 1A4 | DAKO, Glostrup, Denmark | 1:3000 | LSAB/ AP | Steamer |
S-100 protein | Rabbit, polyclonal | DAKO, Glostrup, Denmark | 1:1000 | LSAB/ HRP | - |
Estrogen receptor | mAb 6F11 | Novocastra, Newcastle upon Tyne, UK | 1:200 | LSAB/ AP | Steamer |
Progesterone receptor | mAb 16 | Novocastra, Newcastle upon Tyne, UK | 1:1000 | LSAB/ AP | Steamer |
c-Kit | Rabbit, polyclonal | DAKO, Glostrup, Denmark | 1:100 | LSAB/ AP | Steamer |