Expression of NIS protein in ARO cells expressing different levels of Pax8 gene. Western Blot analysis was performed as described in Methods. In upper panel/autoradiograph of a representative experiment. In lower panel/the staining intensity is expressed as mean ± SD of values obtained from three different experiments. The data are expressed relative to the control values (cells transfected with the empty vector, ARO-pCMV). Statistic analysis was performed using the one-factor ANOVA, followed by t test. P < 0.05 was considered statistically significant.