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Table 1 List of primers for qRT-PCR. Sequence of primers (forward and reverse) used for qRT-PCR experiments. GenBank accession numbers for each gene are indicated in brackets. The primers were designed using an old version of the software Primer 3.0, available at the following website: http://www-genome.wi.mit.edu/cgi-bin/primer/primer3.cgi/

From: Cancer cell adaptation to chemotherapy

Name

Sequence 5'-3' (forward and reverse primer)

Product length

Primer nM

Mg2+ mM

Reference

GAPDH* (NM_002046)

GAA GGT GAA GGT CGG AGT C

226

200

4

-

 

GAA GAT GGT GAT GGG ATT TC

 

200

  

HPRT1* (NM_000194)

TCA GGC AGT ATA ATC CAA AGA TGG T

84

400

4

[38]

 

AGT CTG GCT TAT ATC CAA CAC TTC G

 

400

  

PBGD* (NM_000190)

CTG CAC GAT CCC GAG ACT CT

98

400

4

[39]

 

GCT GTA TGC ACG GCT ACT GG

 

400

  

SDHA* (NM_004168)

TGG GAA CAA GAG GGC ATC TG

 

400

4

[12]

 

CCA CCA CTG CAT CAA ATT CAT G

 

400

  

TBP* (X54993)

CAC GAA CCA CGG CAC TGA TT

89

400

4

[40]

 

TTT TCT TGC TGC CAG TCT GGA C

 

400

  

BCRP (AF098951)

CAC AAC CAT TGC ATC TTG GC

74

400

4

[41]

 

GCT GCA AAG CCG TAA ATC CA

 

400

  

DPD (NM_000110)

CCA AAG GCA GTA AAG CAG GAA

66

400

4

[42]

 

TCA CGA CTC CCC GTA TCG A

 

400

  

EGFR (NM_005228)

TGG TCA AGT GCT GGA TGA TAG A

156

400

4

-

 

GGT AGA AGT TGG AGT CTG TAG GA

 

400

  

ERCC-1 (NM_001983)

GGG AAT TTG GCG ACG TAA TTC

71

400

3

[43]

 

GCG GAG GCT GAG GAA CAG

 

400

  

GST-Ï€ (NM_000852)

CGG AGA CCT CAC CCT GTA

169

400

5

-

 

CGC CTC ATA GTT GGT GTA GA

 

400

  

MDR1 (AF016535)

TGG TTC AGG TGG CTC TGG AT

72

300

4

[44]

 

CTG TAG ACA AAC GAT GAG CTA TCA CA

 

300

  

MLH-1 (NM_000249)

GGC ACA GCA TCA AAC CAA GT

147

400

4

-

 

GCA AGC ATG GCA AGG TCA A

 

400

  

MRP1 (L05628)

CAA TGC TGT GAT GGC GAT G

70

400

4

[39]

 

GAT CCG ATT GTC TTT GCT CTT CA

 

400

  

MRP2 (NM_000392)

TGC AGC CTC CAT AAC CAT GAG

80

400

3

[45]

 

GAT GCC TGC CAT TGG ACC TA

 

400

  

MT-II (NM_005953)

GAT CCC AAC TGC TCC TGC

127

400

4

-

 

ACT TGG CAC AGC CCA CAG

 

400

  

MVP (NM_017458)

CAG CTG GCC ATC GAG ATC A

68

400

4

[39]

 

TCC AGT CTC TGA GCC TCA TGC

 

400

  

TP (NM_001953)

CCT TGG ATA AGC TGG AGT CT

107

400

4

-

 

CCT ACT CTG ACC CAC GAT AC

 

400

  

TS (NM_001071)

CCA GAG ATC GGG AGA CAT GG

66

400

4

-

 

TAC GTG AGC AGG GCG TAG CT

 

400

  

TOPO I (J03250)

CTC CAC AAC GAT TCC CAG AT

149

400

3

-

 

TTA TGT TCA CTG TTG CTA TGC TT

 

400

  

TOPO IIα (NM_001067)

GTA ATT TTG ATG TCC CTC CAC GA

223

400

3

-

 

TCA AGG TCT GAC ACG ACA CTT

 

400

  

TOPO IIβ (NM_001068)

GCA GCC GAA AGA CCT AAA TA

85

400

3

-

 

AAT CAT TAT TGT CAT CAT CAT CAT C

 

400

  
  1. (N.B. Where primer sequences have been taken from references the PCR conditions have been further optimised compared to the original reference.)
  2. * Housekeeping genes.