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Table 1 List of primers for qRT-PCR. Sequence of primers (forward and reverse) used for qRT-PCR experiments. GenBank accession numbers for each gene are indicated in brackets. The primers were designed using an old version of the software Primer 3.0, available at the following website: http://www-genome.wi.mit.edu/cgi-bin/primer/primer3.cgi/

From: Cancer cell adaptation to chemotherapy

Name Sequence 5'-3' (forward and reverse primer) Product length Primer nM Mg2+ mM Reference
GAPDH* (NM_002046) GAA GGT GAA GGT CGG AGT C 226 200 4 -
  GAA GAT GGT GAT GGG ATT TC   200   
HPRT1* (NM_000194) TCA GGC AGT ATA ATC CAA AGA TGG T 84 400 4 [38]
  AGT CTG GCT TAT ATC CAA CAC TTC G   400   
PBGD* (NM_000190) CTG CAC GAT CCC GAG ACT CT 98 400 4 [39]
  GCT GTA TGC ACG GCT ACT GG   400   
SDHA* (NM_004168) TGG GAA CAA GAG GGC ATC TG   400 4 [12]
  CCA CCA CTG CAT CAA ATT CAT G   400   
TBP* (X54993) CAC GAA CCA CGG CAC TGA TT 89 400 4 [40]
  TTT TCT TGC TGC CAG TCT GGA C   400   
BCRP (AF098951) CAC AAC CAT TGC ATC TTG GC 74 400 4 [41]
  GCT GCA AAG CCG TAA ATC CA   400   
DPD (NM_000110) CCA AAG GCA GTA AAG CAG GAA 66 400 4 [42]
  TCA CGA CTC CCC GTA TCG A   400   
EGFR (NM_005228) TGG TCA AGT GCT GGA TGA TAG A 156 400 4 -
  GGT AGA AGT TGG AGT CTG TAG GA   400   
ERCC-1 (NM_001983) GGG AAT TTG GCG ACG TAA TTC 71 400 3 [43]
  GCG GAG GCT GAG GAA CAG   400   
GST-π (NM_000852) CGG AGA CCT CAC CCT GTA 169 400 5 -
  CGC CTC ATA GTT GGT GTA GA   400   
MDR1 (AF016535) TGG TTC AGG TGG CTC TGG AT 72 300 4 [44]
  CTG TAG ACA AAC GAT GAG CTA TCA CA   300   
MLH-1 (NM_000249) GGC ACA GCA TCA AAC CAA GT 147 400 4 -
  GCA AGC ATG GCA AGG TCA A   400   
MRP1 (L05628) CAA TGC TGT GAT GGC GAT G 70 400 4 [39]
  GAT CCG ATT GTC TTT GCT CTT CA   400   
MRP2 (NM_000392) TGC AGC CTC CAT AAC CAT GAG 80 400 3 [45]
  GAT GCC TGC CAT TGG ACC TA   400   
MT-II (NM_005953) GAT CCC AAC TGC TCC TGC 127 400 4 -
  ACT TGG CAC AGC CCA CAG   400   
MVP (NM_017458) CAG CTG GCC ATC GAG ATC A 68 400 4 [39]
  TCC AGT CTC TGA GCC TCA TGC   400   
TP (NM_001953) CCT TGG ATA AGC TGG AGT CT 107 400 4 -
  CCT ACT CTG ACC CAC GAT AC   400   
TS (NM_001071) CCA GAG ATC GGG AGA CAT GG 66 400 4 -
  TAC GTG AGC AGG GCG TAG CT   400   
TOPO I (J03250) CTC CAC AAC GAT TCC CAG AT 149 400 3 -
  TTA TGT TCA CTG TTG CTA TGC TT   400   
TOPO IIα (NM_001067) GTA ATT TTG ATG TCC CTC CAC GA 223 400 3 -
  TCA AGG TCT GAC ACG ACA CTT   400   
TOPO IIβ (NM_001068) GCA GCC GAA AGA CCT AAA TA 85 400 3 -
  AAT CAT TAT TGT CAT CAT CAT CAT C   400   
  1. (N.B. Where primer sequences have been taken from references the PCR conditions have been further optimised compared to the original reference.)
  2. * Housekeeping genes.
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