Global gene expression analysis in time series following N-acetyl L-cysteine induced epithelial differentiation of human normal and cancer cells in vitro

Table 4 Gene ontology for differentially expressed genes found in NHEK cells

Gene Category	Down (genes)	Up (genes)	Combined list Affy probes	EASE score
mitotic cell cycle	41	4	49	1.96E-14
cell cycle	52	12	76	2.03E-12
M phase	22	1	24	7.73E-09
DNA replication and chromosome cycle	22	1	24	7.73E-09
cell proliferation	56	22	100	0.000000024
nuclear division	20	1	22	7.23E-08
M phase of mitotic cell cycle	19	1	21	0.000000217
DNA replication	19	1	21	0.000000217
S phase of mitotic cell cycle	19	1	21	0.000000217
mitosis	19	1	21	0.000000217
DNA metabolism	29	6	41	0.000000403
DNA dependent DNA replication	13	0	13	0.00000641
cytokinesis	12	0	12	0.0000199
metabolism	100	79	256	0.000126
regulation of cell cycle	26	11	48	0.00137
cell growth and/or maintenance	79	62	202	0.00241
nucleobase\, nucleoside\, nucleotide and nucleic acid metabolism	53	36	124	0.00262
biosynthesis	15	5	24	0.00475
protein metabolism	43	29	101	0.00992
physiological process	132	130	389	0.0103
obsolete biological process	17	7	31	0.0125
macromolecule biosynthesis	11	4	18	0.0267
protein modification	22	14	50	0.0639
cytoplasm organization and biogenesis	12	6	23	0.0693
cell organization and biogenesis	17	13	42	0.211
phosphorus metabolism	16	12	40	0.244
phosphate metabolism	16	12	40	0.244
protein amino acid phosphorylation	12	9	30	0.326
phosphorylation	12	10	32	0.424
macromolecule catabolism	10	8	26	0.435
protein catabolism	10	8	26	0.435
proteolysis and peptidolysis	10	8	26	0.435
cell adhesion	4	16	36	1
cell motility	2	6	14	0.995

Bold: processes significantly affected in Caco-2 pointing to differentiation
Italicized: processes significantly downregulated in NHEK, pointing to differentiation
Cutoff (EASE score < 5.00E-01)
Cutoff (#overlapping genes > 9, larger than
in Caco-2 due to a large initial list size).

ISSN: 1471-2407