Table 3 Relative gene expression levels measured by real time kinetic PCR in Caco-2 and NHEK cells after NAC treatment as compared to untreated cells.
Caco-2 | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
Gene | Common | Genbank | 1h | 12h | 24h | 48h | Â | Â | ||||
| Â | Â | Â | RT-PCR | Affymetrix | RT-PCR | Affymetrix | RT-PCR | Affymetrix | RT-PCR | Affymetrix | Â | Â |
matrix metalloproteinase 9* | MMP9 | J05070 | na | nd | no change | up | na | up | na | na | Â | Â |
aldo-keto reductase* | AKR1C3 | D17793 | na | nd | no change | up | up (2.8) | nd | na | na | Â | Â |
aquaporin 3* | AQP3 | N74607 | no change | down | up (6.8) | up | up (5.0) | up | na | na | Â | Â |
plasminogen activator, tissue* | PLAT | M15518 | na | nd | no change | nd | no change | nd | na | na | Â | Â |
HMG-box transcription factor 1* | HBP1 | AF019214 | no change | nd | up (2.6) | up | up (2.1) | nd | na | na | Â | Â |
Cox-2* | PTGS2 | U04636 | no change | nd | no change | nd | down (2.2) | down | na | na | Â | Â |
v-erb-b2* | ERBB3 | M34309 | na | nd | up (3.7) | up | no change | nd | na | na | Â | Â |
proline-rich nuclear receptor coactivator 1* | PNRC1 | U03105 | na | nd | no change | up | up (2.4) | nd | na | na | Â | Â |
inhibitor of differentiation* | ID1 | AA457158 | down (3.8) | down | na | nd | na | nd | na | nd | Â | Â |
E-cadherin** | CDH1 | Z13009 | na | nd | no change | nd | up | nd | no change | na | Â | Â |
hsp27** | HSPB1 | U90906 | na | nd | up | nd | up | nd | no change | na | Â | Â |
p53** | TP53 | U94788 | na | nd | up | nd | no change | nd | no change | na | Â | Â |
N-myc downstream regulated gene 1** | NDRG1 | D87953 | na | nd | up | nd | up | up | up | na | Â | Â |
NHEK | ||||||||||||
| Â | Â | Â | 30 min | 3h | 12h | 24h | 48h | |||||
Gene | Common | Genbank | RT-PCR | Affymetrix 1 h | RT-PCR | Affymetrix | RT-PCR | Affymetrix | RT-PCR | Affymetrix | RT-PCR | Affymetrix |
matrix metalloproteinase 9* | MMP9 | J05070 | no change | nd | no change | nd | na | up | na | up | up (11.1) | nd |
aldo-keto reductase* | AKR1C3 | D17793 | na | nd | down (4.0) | nd | na | nd | na | nd | down (5.3) | nd |
aquaporin 3* | AQP3 | N74607 | no change | nd | no change | nd | na | nd | na | up | up (2.8) | nd |
plasminogen activator, tissue* | PLAT | M15518 | no change | nd | no change | nd | na | nd | na | down | no change | nd |
HMG-box transcription factor 1* | HBP1 | AF019214 | down (6.3) | nd | down (4.0) | nd | na | up | na | nd | no change | nd |
Cox-2* | PTGS2 | U04636 | down (7.7) | nd | down (6.3) | nd | na | nd | na | down | down (8.3) | nd |
v-erb-b2* | ERBB3 | M34309 | na | nd | down (2.4) | nd | na | up | na | nd | down (4.5) | nd |
proline-rich nuclear receptor coactivator 1* | PNRC1 | U03105 | na | nd | down (5.3) | nd | na | up | na | nd | no change | nd |
inhibitor of differentiation* | ID1 | AA457158 | down (8.3) | down | na | nd | na | nd | na | nd | na | nd |
E-cadherin** | CDH1 | Z13009 | na | nd | na | nd | no change | nd | up | nd | up | nd |
hsp27** | HSPB1 | U90906 | na | nd | na | nd | up | nd | up | nd | up | nd |
p53** | TP53 | U94788 | na | nd | na | nd | no change | nd | up | nd | up | nd |
N-myc downstream regulated gene 1** | NDRG1 | D87953 | na | nd | na | nd | no change | nd | no change | nd | no change | nd |