Figure 5

Multiple mechanisms of the transcriptional repression of the hTR gene by mdm2. Schematically shown are various mechanisms by which mdm2 might act on the hTR core promoter. The hTR core promoter elements include Sp1 sites (recognised by Sp1 family factors) and a CCAAT-box (recognized by NF-YA, B and C). NF-Y may facilitate the access of upstream activators (such as Sp1) to their cognate enhancer/promoter sequences, recruit TAFs components of TFIID [33] and serve to instigate the formation of a pre-initiation complex of general transcription factors and Pol II. Co-regulators such as pRB/mdm2 for modulation of signals between transcription factors and the core transcriptional machinery might act through the hTR core promoter directly or indirectly.