Table 1 Primers used to amplify the VHL gene
Name | Sequence 5'>3' | Exon | Tm | Fragment length |
|---|---|---|---|---|
Sense3a | GGT CTG GAT CGC GGA GGG A | 1 | 64°C * | 191 bp |
Asense4a | GCC CGG CCT CCA TCT CCT | 1 | Â | Â |
Sense5a | AGT CGG GCG CCG AGG AGT | 1 | 64°C * | 184 bp |
Asense6a | CCG TCG AAG TTG AGC CAT AC | 1 | Â | Â |
Sense7a | CCC AGG TCA TCT TCT GCA AT | 1 | 64°C †| 159 bp |
Asense8a | CTG CTG GGT CGG GCC TAA G | 1 | Â | Â |
Sense9 | GTG GCT CTT TAA CAA CCT TTG C | 2 | 60°C‡ | 194 bp |
Asense10 | CCT GTA CTT ACC ACA ACA ACC TTA TC | 2 | Â | Â |
Sense11a | CAC TGA GGA TTT GGT TTT TGC | 3 | 55°C | 162 bp |
Asense12a | TCC AGG TCT TTC TGC ACA TTT | 3 | Â | Â |
Sense13a | GAC ATC GTC AGG TCG CTC TA | 3 | 55°C | 150 bp |
Asense14a | TCA AAA GCT GAG ATG AAA CAG TG | 3 | Â | Â |