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Table 1 Primers used to amplify the VHL gene

From: Prevalence of von Hippel-Lindau gene mutations in sporadic renal cell carcinoma: results from the Netherlands cohort study

Name Sequence 5'>3' Exon Tm Fragment length
Sense3a GGT CTG GAT CGC GGA GGG A 1 64°C * 191 bp
Asense4a GCC CGG CCT CCA TCT CCT 1   
Sense5a AGT CGG GCG CCG AGG AGT 1 64°C * 184 bp
Asense6a CCG TCG AAG TTG AGC CAT AC 1   
Sense7a CCC AGG TCA TCT TCT GCA AT 1 64°C † 159 bp
Asense8a CTG CTG GGT CGG GCC TAA G 1   
Sense9 GTG GCT CTT TAA CAA CCT TTG C 2 60°C‡ 194 bp
Asense10 CCT GTA CTT ACC ACA ACA ACC TTA TC 2   
Sense11a CAC TGA GGA TTT GGT TTT TGC 3 55°C 162 bp
Asense12a TCC AGG TCT TTC TGC ACA TTT 3   
Sense13a GAC ATC GTC AGG TCG CTC TA 3 55°C 150 bp
Asense14a TCA AAA GCT GAG ATG AAA CAG TG 3   
  1. * 3% DMSO added for PCR
  2. † 1.5% DMSO and 2.5 mM MgCl2 added for PCR
  3. ‡ 4% DMSO and 1.0 mM MgCl2 added for PCR