Figure 5
From: Reevaluation of the 22-1-1 antibody and its putative antigen, EBAG9/RCAS1, as a tumor marker

Endogenous expression of EBAG9 in cancer cell lines. Cells were lysed in NP-40 buffer and 50 μg of total protein was separated by SDS-PAGE and analysed by immunoblot using our polyclonal anti-EBAG9 antibody. α-actin served as control indicating protein load.