Figure 1

SAOSp cells undergo anoikis after culture under suspended conditions. After culture under suspended conditions for 24 h SAOSp and SAOSar cell viability was assayed using a Live/Dead™ assay. Panel A shows a larger fraction of SAOSp cells stained red (dead) than of SAOSar cells (majority stained green). Apoptosis was quantitated by PI (panel B) or Annexin V-FITC/PI (panel C) staining followed by flow cytometry analyses. Panel B shows a higher percentage of SAOSp (33.3%) cells than of SAOSar (8.37%) in the sub-G/0 phase representative of apoptotic cells. Similar results are shown in panel C, in which a higher percentage of SAOSp cells (32.6%) than of SAOSar (6.09%) stained positive for Annexin V (top and bottom right quadrants) representative of apoptotic cells. Results shown are representative of three independent experiments.