Human desmoid fibroblasts: matrix metalloproteinases, their inhibitors and modulation by Toremifene

BMC Cancer

Table 3 ³H-proline incorporation into collagen fibres

	Cells	Media	Cells + Media
	48 h
Desmoid fibroblasts	6,852 ± 290	33,915 ± 2,307	40,767
Desmoid fibroblasts + toremifene	4,243 ± 361*	25,097 ± 814*	29,340
	24 h
Desmoid fibroblasts	4,252 ± 416	15,423 ± 1,100	19,675
Desmoid fibroblasts + toremifene	1,432 ± 101*	9,221 ± 381*	10,653
	3 h
Desmoid fibroblasts	1,176 ± 48	1,498 ± 56	2,674
Desmoid fibroblasts + toremifene	534 ± 31*	745 ± 74*	1,279

Fibroblasts were cultured for 48 h in MEM with or without toremifene. ³H-proline was added for 48 h, for the last 24 h and for the last 3 h. Total radioactivity value is expressed in cpm/mg protein and represents the mean ± SD of three determinations, each in quadruplicate. The statistical analysis was performed with analysis of variance (ANOVA). Differences vs desmoid fibroblasts: F-test significant at *99%.

ISSN: 1471-2407