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Table 1 Correlation of mouse S100A7/psoriasin expression using RT-PCR and in situ hydridization in normal mammary gland and DMBA-induced mammary tumors Mouse S100A7/psoriasin mRNA levels were determined in extracts from frozen tissue samples of matched normal mammary gland and DMBA-induced mammary tumors (n = 6) by semi-quantitative RT-PCR as described in Materials and Methods. Sections from the corresponding adjacent tissue samples which had been formalin fixed and paraffin-embedded, where used for in situ hybridization (ISH) with 35S-labelled antisense mouse S100A7/psoriasin probes. Specific hybridization was scored on a scale of 0 to 3 as described in Materials and Methods. Results of the two analyses were correlated using Spearmans test (r = 0.63, p < 0.02).

From: Expression analysis of the mouse S100A7/psoriasin gene in skin inflammation and mammary tumorigenesis

   Relative mouse S100A7/psoriasin expression
  Sample RT-PCR ISH
Normal MG 1 0.37 0
  2 0.16 0
  3 0.14 0
  4 0.21 <0.5
  5 0.73 <0.5
  6 0.42 0
MG Tumor 1 1.50 2
  2 1.63 3
  3 1.12 2
  4 0.22 1
  5 1.22 1
  6 0.13 1