Skip to main content

Table 1 Correlation of mouse S100A7/psoriasin expression using RT-PCR and in situ hydridization in normal mammary gland and DMBA-induced mammary tumors Mouse S100A7/psoriasin mRNA levels were determined in extracts from frozen tissue samples of matched normal mammary gland and DMBA-induced mammary tumors (n = 6) by semi-quantitative RT-PCR as described in Materials and Methods. Sections from the corresponding adjacent tissue samples which had been formalin fixed and paraffin-embedded, where used for in situ hybridization (ISH) with 35S-labelled antisense mouse S100A7/psoriasin probes. Specific hybridization was scored on a scale of 0 to 3 as described in Materials and Methods. Results of the two analyses were correlated using Spearmans test (r = 0.63, p < 0.02).

From: Expression analysis of the mouse S100A7/psoriasin gene in skin inflammation and mammary tumorigenesis

  

Relative mouse S100A7/psoriasin expression

 

Sample

RT-PCR

ISH

Normal MG

1

0.37

0

 

2

0.16

0

 

3

0.14

0

 

4

0.21

<0.5

 

5

0.73

<0.5

 

6

0.42

0

MG Tumor

1

1.50

2

 

2

1.63

3

 

3

1.12

2

 

4

0.22

1

 

5

1.22

1

 

6

0.13

1