Figure 4

Cell growth curves of cell lines SK-N-AS, 293 and K562 after transfection of in vitro transcribed mRNA and monitoring for 4 days after transfection. The average number of cells in 8 separate experiments is shown at each time point for each construct. Standard deviation is indicated with a bar. A: Two different amounts of mRNA have been used for ENO1-mRNA: 1.5 (1.5 μg ENO1-mRNA) or 0.3 (0.3 μg ENO1-mRNA + 1.2 μg EGFP-mRNA), compared to 1.5 μg EGFP-mRNA and lipofectamine only. There is strong statistical significance (p << 0.001) for difference in cell numbers between the ENO1-mRNA-experiments and the EGFP-mRNA experiments in SK-N-AS and 293-cells (exact p-values for each day are listed in Table 3), in K562 cells the difference was not significant. B: Cell growth curves of SK-N-AS cells after transfection of 1.5 μg of ENO1, ENO1-mut and MBP-1 mRNAs and monitoring for 4 days. The difference in cell numbers between ENO1 and MBP-1 was significant for day 2–4 (p = 0.02), ENO1 and ENO1-mut did not significantly differ. C: Cell growth curves of SK-N-AS cells after transfection of 0.3 μg ENO1, ENO1-mut and MBP-1 mRNAs and monitoring for 4 days. The difference in cell numbers between ENO1 and MBP-1 was significant for day 2 (p = 0.006), 3 (p = 0.001) and 4 (p = 0.02). ENO1 and ENO1-mut did not significantly differ.